Purification of Extracellular Collagenase from Pseudomonas sp: Remarkable Collagenolytic Activity
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چکیده
منابع مشابه
Purification and Characterization of 50 kDa Extracellular Metalloprotease from Serratia sp. ZF03
Background: Proteolytic enzymes have an important role in variety of physiological and pathological functions. They have been used in therapeutic and pharmaceutical applications. Characterizations of extracellular proteases from various strains of S. marcescens indicate that most strains produce a very similar major metalloprotease. This metalloprotease (serrapeptidase, serrapeptase) is an impo...
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Psychrotrophic Pseudomonas ADT3 (NCBI GenBank Acc.no.JX914667) is capable of growth on lipid as the sole carbon source. In this paper, we report the purification and characterization of an extracellular lipase from psychrotroph, isolated from soil sample of NyAlesund, Svalbard, Arctic region. The Pseudomonas ADT3 isolate produces lipase enzyme in the extracellular minimal media with only 1% oli...
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A novel neutrophilic metalloprotease was isolated from Pseudomonas sp. DR89 isolate which was identified ina mineral spring in Iran. The enzyme was purified from the isolate to 21-folds in a three-step procedure involving ammonium sulfate precipitation, Q-Sepharose ionic exchange and Sephadex G-100 gel filtrationchromatography. Resuts showed that the enzyme was active at high temper...
متن کاملEXTRACELLULAR LIPA SE ACTIVITY CHARACTERIZATION OF SOME PSEUDOMONAS AERUGINOSA STRA INS ISOLATED FROM HUMAN INFECTIONS
Pseudomonas aeruginosa EF2, ATCC 9027 and ATCC 19660 were grown in a continuous culture under Tween 80 (polyoxyethylene sorbitan monooleate) limitation and optimum conditions (pH 6.5, 37°C at dilution rate of 0.05/h). Culture supernatants were carefully removed and stored at -20°C. To purify the lipases, the culture supernatant was reduced in volume to approximately 10 mL by an ultrafiltra...
متن کاملExtracellular collagenase from Trichophyton schoenleinii.
The dermatophyte Trichophyton schoenleinii elaborates an extracellular collagenase which is specific for native collagen and gelatin and is separable from an enzyme which is active against Azocoll. The fungal enzyme differs from the bacterial collagenases in its acid pH and small molecular weight. In addition, the fungal enzyme is irreversibly inhibited by ethylenediaminetetraacetic acid, where...
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ژورنال
عنوان ژورنال: Advances in Biotechnology & Microbiology
سال: 2017
ISSN: 2474-7637
DOI: 10.19080/aibm.2017.04.555633